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IMPROVEMENT OF GENE TARGETING EFFICIENCY BY DNA CHEMICAL MODIFICATION |
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Principal Investigator: M. BICHARA
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TECHNICAL DESCRIPTION
This invention describes a process to increase gene targeting frequency in E. coli (factor 1 000 to 100 000). Chemical modifications result in DNA damage, blocking of DNA replication and improvement of homologous recombination efficiency.
This process allows gene targeting by integrating a modified gene in place of a healthy gene for the inactivation of said gene.
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