CONTEXT

Randomised mutagenesis of genes of interest is nowadays commonly used to solve molecular genetic engineering problems. In the existing prior art, the method consists in (1) the generation of a library of mutated genes and (2) the screening of this library for the presence of mutants possessing a given property, either in vitro or in bacterial cells, if possible.

However, when eukaryotic proteins are targeted, the properties observed in vitro for a given mutant, often do not result in the desired phenotype when introduced in eukaryotic cells. There is thus a need for a simple and effective method to create genetic diversity, adapted to mammalian or human cell applications.