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DIRECTED EVOLUTION OF GENOMIC REGIONS BASED ON LENTIVIRAL VECTORS |
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Principal investigator : Matteo NEGRONI
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CONTEXT
Randomised mutagenesis of genes of interest is nowadays commonly used to solve molecular genetic engineering problems. In the existing prior art, the method consists in (1) the generation of a library of mutated genes and (2) the screening of this library for the presence of mutants possessing a given property, either in vitro or in bacterial cells, if possible.
However, when eukaryotic proteins are targeted, the properties observed in vitro for a
given mutant, often do not result in the desired phenotype when introduced in
eukaryotic cells. There is thus a need for a simple and effective method to create genetic diversity, adapted to mammalian or human cell applications.
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