|
RHO-GEF FAMILY AS DRUG TARGETS FOR AXON REGENERATION |
|
Inventors: A. DEBANT and S. SCHMIDT
|
TECHNICAL DESCRIPTION
Using the screening test described below, the researchers isolated a peptide inhibitor that targets the second RhoGEF domain of the protein Trio. This inhibitor is highly specific and protects neuronal cells from serum-induced neurite retraction, indicating that this inhibitor has potential applications in axon regeneration.
The scientists used an aptamer library to determine inhibitors of the proteins from the Rho-GEF family by a screening process that includes:
i. recovering peptide aptamers that bind to the protein of interest;
ii. recovering the peptides that specifically inhibit the in vitro exchange activity of the GEF domain of said protein;
iii. testing the capacity of the selected peptides to inhibit in intact cells the activity of the RhoGEF on the appropriate GTPase.
This RhoGEF inhibitor could have potential applications in different disease processes, such as nerve regeneration or cancer.
Pour lire la suite, merci de vous identifier ou de vous inscrire dans la zone de droite... |
