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COMPOUNDS FOR THE DETECTION THE QUANTIFICATION OF CELL APOPTOSIS |
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Y. MELY, A. KLYMCHENKO, O. DEMCHENKO and V. SHYNKAR.
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TECHNICAL DESCRIPTION
Cell apoptosis (programmed death cell), which is of critical importance in cancers, results in changes of the lipid composition of the outer leaflet of the cell plasma membrane.
The present invention describes a new probe for the detection of apoptotic cells. This new fluorescent probe labels the outer leaflet of the cell membrane and responds to apoptotic changes in lipid composition by variation of the relative intensities of its two emission bands.
In comparison with the commonly used Annexin V-based fluorescent kit, this probe provides: (1) faster sample preparation and (2) a ratiometric detection, which allows for a more precise quantification of apoptosis by fluorescence microscopy, cytometry, or spectroscopy applications.
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