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USE OF A CRYSTAL COMPRISING DNA POLYMERASE CLAMP FACTOR FOR THE CONCEPTION OF NEW ANTIBIOTICS |
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Principal Investigator: D. BURNOUF
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TECHNICAL DESCRIPTION
A successful DNA replication process requires that the DNA polymerase becomes processive through its association, via a short peptide, with a processivity factor. A new approach is being developed to inhibit bacterial DNA replication by impeding this interaction. It relies on the co-crystallization of a complex containing the Escherichia coli processivity factor beta and the binding peptide from an E. coli DNA polymerase.
The present invention relates to the crystallization method, the crystal structure and the use of the atomic coordinates for the screening and the design of new antibacterial drugs.
Ref.: Structural and biochemical analysis of sliding clamp/ligand interactions suggest a competition between replicative and translesion DNA polymerases. Burnouf DY, Olieric V, Wagner J, Fujii S, Reinbolt J, Fuchs RP, Dumas P. J Mol Biol. 2004 Jan 30;335(5):1187-97
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