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METHOD FOR SCREENING ANTICANCER SUBSTANCES SET OR KIT FOR IMPLEMENTING SAID METHOD |
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Principal Investigator: Pierre ROUX
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TECHNICAL DESCRIPTION
The inventors have developed a new method for screening anticancer substances. This protocol is based on the colon cancer model cells SW620 and the observation that neoformation of E-cadherin type junctions is in correlation with metastases reduction.
This protocol includes the following steps:
a) obtaining a culture of tumoral cells not expressing E-cadherin on their cell membrane
b) contacting the cells obtained at step a) with a candidate substance or a combination of candidate substances
c) detecting, in the culture of cells obtained at the end of step b), the presence of E-cadherin at the cell surface
d) positively selecting the candidate substance or combination of candidate substances when E-cadherin has been detected at step c).
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